细粒棘球绦虫原头蚴lncRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2019.09.014

Abstract

Abstract: The purpose of this study was to analyze the expression profile characteristics of lncRNA of the protoscoleces in Echinococcus granulosus and to provide theoretical evidence for further revealing the role of lncRNA in the development of the protoscoleces. The protoscoleces were collected from the infected sheep liver under sterile condition, and the total RNA was extracted. Then the lncRNA Library of the protoscoleces was constructed and sequenced by RNA sequencing technology. The results showed that 609 novel lncRNA molecules were identified from the protoscoleces for the first time. Among them, there are 431 intergenic lncRNAs, 11 intronic lncRNAs, 68 anti-sense lncRNAs, and 99 sence lncRNAs. GO analysis indicated that the top 200 lncRNA molecules were mainly associated with negative regulation of MAP kinase, SMC binding complex, IMP biosynthesis, and acetyltransferase activator activity. The results of KEGG analysis indicated that the top 200 lncRNA molecules were mainly associated with apoptosis, steroid skeleton biosynthesis, carbon metabolism, and dorsal-ventral axis formation. The results of cis analysis indicated that in these 609 novel lncRNA molecules, there are 79, 31, 10, 128, 43, 84 and 73 lncRNA moleculess potential interplayed with the Wnt signaling pathway, Hedgehog signaling pathway, NF-κB signaling pathway, cAMP signaling pathway, Notch signaling pathway, bile-salt signaling pathway, and dorsal-ventral axis. The results of qRT-PCR showed that the top 10 lncRNA molecules were consistent with the sequencing results. The results of whole-mount in situ hybridization showed that the highly expressed lncRNA molecules were expressed in the sucker, epidermis and disperse cells of the protoscoleces. To sum up, a large number of novel lncRNA molecules were found in the E. granulosus in this study, which enriched the data of lncRNA of the protoscoleces, and laid a foundation for further analysis of the role of lncRNA in the development of protoscoleces in E. granulosus.

CLC Number:

S852.734

WANG Zhengrong, ZHANG Yanyan, MA Xun, LIU Yi, XU Mengfei, MENG Jimeng, BO Xinwen. lncRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1864-1873.

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lncRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

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